Enabling automated cell preparation by controlled volume dispensing
In modern laboratory workflows, precise cell preparation is essential for applications ranging from basic research to advanced drug development. Traditionally a manual and time-consuming task, recent advancements have enabled automation through controlled volume dispensing using microfluidic technologies. This case study highlights the use of the mp6 micropump by Bartels Mikrotechnik to automate this process with a compact and easy-to-set-up system. The pump is paired with a flow sensor for real-time volume feedback and software-based control, allowing for targeted dispensing of defined fluid volumes.
Experiments showed that for target volumes of 1000 µl and 100 µl, the system performed reliably with minimal deviation. However, as volumes decreased to 10 µl and 1 µl, deviations increased significantly. This indicates current limitations in achieving precision at lower scales, likely due to the flow integration software and sensor sampling rate. Nonetheless, by adjusting pump parameters and improving software algorithms, the system shows promise for enhanced microfluidic control.
A practical demonstration also integrated the micropump into an automated robotic system for well plate filling and cell culture preparation, underlining its potential in scalable, high-throughput lab automation. Its small size, reliability, and use of off-the-shelf components make it a strong candidate for future optimization. Continued development will focus on improving dosing precision at sub-microliter levels. A video demonstration of the setup is available on YouTube: https://www.youtube.com/watch?v=WdiGUPV8eVY.